細胞增殖WST-8檢測試劑 50mM水溶液

貨號15707存儲條件 在2-8度冷藏保存, 避免光照
規格1 g價格9744
Ex (nm)460Em (nm)
分子量600.5溶劑Water
產品詳細介紹


簡要概述

產品基本信息

貨號:15707

產品名稱:細胞增殖WST-8檢測試劑 50mM水溶液 

規格:1g

儲存條件:-15℃避光防潮

保質期:12個月

 

產品物理化學光譜特性

分子量:N/A

溶劑:水

激發波長(nm):460

發射波長(nm):N/A

 

產品介紹

細胞增殖WST-8檢測試劑 50mM水溶液是美國AAT Bioquest生產的用于檢測細胞增殖的試劑,WST-8(2-(2-甲氧基-4-硝基苯基)-3-(4-硝基苯基)-5-(2,4-二磺基苯基)-2H-四唑鹽,單鈉鹽)是一種水溶性四唑鹽,用于 評估細胞代謝活動。 在中性pH和中間電子受體存在下,1-甲氧基吩嗪硫酸甲酯,NADPH依賴性細胞氧化還原酶,通過質膜電子傳遞,將細胞外無色細胞不透性WST-8還原為水溶性橙 甲dye染料,最大吸收波長為460 nm。 WST-8非常穩定,由于其細胞毒性很小,因此可以耐受長時間的孵育(例如24至48小時)。 WST-8允許靈敏的比色測定法測定增殖和細胞毒性測定中活細胞的數量。 檢測靈敏度高于任何其他四唑鹽,如MTT,XTT或MTS。 它通常用作細胞增殖測定中的細胞活力指示劑。百螢生物是AAT Bioquest的中國代理商,為您提供最優質的細胞增殖WST-8檢測試劑 50mM水溶液。 

 

參考文獻

miR-218 inhibits the proliferation of human glioma cells through downregulation of Yin Yang 1
Authors: Gao, Yong and Sun, Laisheng and Wu, Zicheng and Xuan, Chengmin and Zhang, Junxia and You, Yongping and Chen, Xincheng
Journal: Molecular Medicine Reports (2017)

A sensitive WST-8-based bioassay for PEGylated granulocyte colony stimulating factor using the NFS-60 cell line
Authors: Tiwari, K.; Wavdhane, M.; Haque, S.; Govender, T.; Kruger, H. G.; Mishra, M. K.; Ch and ra, R.; Tiwari, D.
Journal: Pharm Biol (2015): 849-54

Comparison of the WST-8 colorimetric method and the CLSI broth microdilution method for susceptibility testing against drug-resistant bacteria
Authors: Tsukatani, T.; Suenaga, H.; Shiga, M.; Noguchi, K.; Ishiyama, M.; Ezoe, T.; Matsumoto, K.
Journal: J Microbiol Methods (2012): 160-6

WST-8 analysis of cell viability during osteogenesis of human mesenchymal stem cells
Authors: Stoddart, M. J.
Journal: Methods Mol Biol (2011): 21-5

monosodium salt (WST-8) rapid colorimetric assays for antimicrobial susceptibility testing of staphylococci and ESBL-producing clinical isolates
Authors: Brady, A. J.; Kearney, P.; Tunney, M. M., Comparative evaluation of 2,3-bis [2-methyloxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide (XTT) and 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2, 4-disulfophenyl)-2H-tetrazolium
Journal: J Microbiol Methods (2007): 305-11

Reactivity of blood samples spotted onto filter papers in the WST-8 method for screening of G6PD deficiency
Authors: Arai, M.; Kosuge, K.; Kawamoto, F.; Matsuoka, H.
Journal: Acta Med Okayama (2006): 127-34



說明書
細胞增殖WST-8檢測試劑 50mM水溶液 .pdf


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