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Cell Navigator?TMR神經酰胺高爾基體染色試劑盒*紅色熒光*

貨號22752存儲條件 在零下15度以下保存, 避免光照
規格100 Tests價格3648
Ex (nm)544Em (nm)570
分子量溶劑
產品詳細介紹


簡要概述

高爾基體是大多數真核細胞胞質內囊泡和折疊膜的復合體,參與分泌和細胞內運輸。它修飾內質網(ER)中內置的蛋白質和脂質,并準備將其運輸到細胞外。它還在脂質在整個細胞中的運輸和溶酶體的形成中起著重要作用。Cell Navigator™TMR神經酰胺高爾基染色試劑盒提供了一種簡便,快速的方法,可以在活細胞中以紅色熒光染色高爾基體。高爾基體通過形成相應的熒光代謝產物而被染色。Cell Navigator™TMR神經酰胺高爾基染色試劑盒提供了一種優化的測定方法,可用于通過熒光顯微鏡檢查高爾基體的形態。

點擊查看光譜

 

適用儀器


熒光顯微鏡  
激發: Cy3/TRITC濾波片組
發射: Cy3/TRITC濾波片組
推薦孔板: 黑色透明
 


產品說明書

樣品分析方案

概述

根據需要處理細胞

加入GGR169-神經酰胺工作溶液并在室溫或37°C下孵育15至30分鐘

添加染色緩沖液

使用Cy3濾波片組在顯微鏡下觀察 

 

儲備溶液配制

GGR169-神經酰胺原液(100X)
向GGR-神經酰胺(組分A)中加入100 µL DMSO(組分C)制成GGR169-神經酰胺原液(100X)。
注意:將未使用的GGR-神經酰胺原液分裝在-20°C下,以單次使用,避免凍融循環。
 
工作溶液配制
GGR169-神經酰胺工作液
將10 µL GGR169-神經酰胺原液(100X)添加到990 µL染色緩沖液(組分B)中,制成GGR169-神經酰胺工作液。
可選:向1 mL GGR169-神經酰胺工作溶液中加入10 µL Hoechst 33342(組分D)以進行核染色。在帶有DAPI濾光片組的熒光顯微鏡下觀察。

 

操作步驟

以下指南應用于指導方針,并可根據要求進行修改。
  1. 接種并根據需要處理細胞。
  2. 將100 µL GGR169-神經酰胺工作溶液直接添加到細胞培養基中。
  3. 在室溫或37°C下孵育15至30分鐘。
  4. 除去GGR169-神經酰胺工作溶液,并用DPBS或您選擇的緩沖液清洗一次。
  5. 加入100 µL /孔的染色緩沖液(組分B)。
  6. 在裝有Cy3濾光片的熒光顯微鏡下觀察。 

 

圖示

圖1.HeLa細胞中GGR169神經酰胺高爾基染色的熒光圖像。在37°C下,用100μL具有Hoechst 33342的GGR169-神經酰胺工作溶液對細胞染色20分鐘。

 

參考文獻

Chlamydia trachomatis-infected human cells convert ceramide to sphingomyelin without sphingomyelin synthases 1 and 2.
Authors: Tachida, Yuriko and Kumagai, Keigo and Sakai, Shota and Ando, Shuji and Yamaji, Toshiyuki and Hanada, Kentaro
Journal: FEBS letters (2020): 519-529

The role of ceramide in regulating endoplasmic reticulum function.
Authors: Zelnik, Iris D and Ventura, Ana E and Kim, Jiyoon L and Silva, Liana C and Futerman, Anthony H
Journal: Biochimica et biophysica acta. Molecular and cell biology of lipids (2020): 158489

Neuronal ceroid lipofuscinosis related ER membrane protein CLN8 regulates PP2A activity and ceramide levels.
Authors: Adhikari, Babita and De Silva, Bhagya and Molina, Joshua A and Allen, Ashton and Peck, Sun H and Lee, Stella Y
Journal: Biochimica et biophysica acta. Molecular basis of disease (2019): 322-328

Structure, functions and regulation of CERT, a lipid-transfer protein for the delivery of ceramide at the ER-Golgi membrane contact sites.
Authors: Kumagai, Keigo and Hanada, Kentaro
Journal: FEBS letters (2019): 2366-2377

Activation of neutral sphingomyelinase 2 by starvation induces cell-protective autophagy via an increase in Golgi-localized ceramide.
Authors: Back, Moon Jung and Ha, Hae Chan and Fu, Zhicheng and Choi, Jong Min and Piao, Yongwei and Won, Jong Hoon and Jang, Ji Min and Shin, In Chul and Kim, Dae Kyong
Journal: Cell death & disease (2018): 670

Antidepressants act by inducing autophagy controlled by sphingomyelin-ceramide.
Authors: Gulbins, Anne and Schumacher, Fabian and Becker, Katrin Anne and Wilker, Barbara and Soddemann, Matthias and Boldrin, Francesco and Müller, Christian P and Edwards, Michael J and Goodman, Michael and Caldwell, Charles C and Kleuser, Burkhard and Kornhuber, Johannes and Szabo, Ildiko and Gulbins, Erich
Journal: Molecular psychiatry (2018): 2324-2346

Both the N- and C- terminal regions of the Chlamydial inclusion protein D (IncD) are required for interaction with the pleckstrin homology domain of the ceramide transport protein CERT.
Authors: Kumagai, Keigo and Elwell, Cherilyn A and Ando, Shuji and Engel, Joanne N and Hanada, Kentaro
Journal: Biochemical and biophysical research communications (2018): 1070-1076

Ceramide Transporter CERT Is Involved in Muscle Insulin Signaling Defects Under Lipotoxic Conditions.
Authors: Bandet, Cécile L and Mahfouz, Rana and Véret, Julien and Sotiropoulos, Athanassia and Poirier, Maxime and Giussani, Paola and Campana, Mélanie and Philippe, Erwann and Blachnio-Zabielska, Agnieszka and Ballaire, Raphaëlle and Le Liepvre, Xavier and Bourron, Olivier and Berkeš, Dušan and Górski, Jan and Ferré, Pascal and Le Stunff, Hervé and Foufelle, Fabienne and Hajduch, Eric
Journal: Diabetes (2018): 1258-1271

Ceramide-transfer protein-mediated ceramide transfer is a structurally tunable flow-inducing mechanism with structural feed-forward loops.
Authors: Giordano, Giulia
Journal: Royal Society open science (2018): 180494

Phosphoinositide binding by the PH domain in ceramide transfer protein (CERT) is inhibited by hyperphosphorylation of an adjacent serine-repeat motif.
Authors: Sugiki, Toshihiko and Egawa, Daichi and Kumagai, Keigo and Kojima, Chojiro and Fujiwara, Toshimichi and Takeuchi, Koh and Shimada, Ichio and Hanada, Kentaro and Takahashi, Hideo
Journal: The Journal of biological chemistry (2018): 11206-11217



說明書
Cell Navigator?TMR神經酰胺高爾基體染色試劑盒*紅色熒光*.pdf


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