Cell Navigator?TMR神經酰胺高爾基體染色試劑盒*紅色熒光*

貨號22752存儲條件 在零下15度以下保存, 避免光照
規格100 Tests價格3648
Ex (nm)544Em (nm)570


高爾基體是大多數真核細胞胞質內囊泡和折疊膜的復合體,參與分泌和細胞內運輸。它修飾內質網(ER)中內置的蛋白質和脂質,并準備將其運輸到細胞外。它還在脂質在整個細胞中的運輸和溶酶體的形成中起著重要作用。Cell Navigator™TMR神經酰胺高爾基染色試劑盒提供了一種簡便,快速的方法,可以在活細胞中以紅色熒光染色高爾基體。高爾基體通過形成相應的熒光代謝產物而被染色。Cell Navigator™TMR神經酰胺高爾基染色試劑盒提供了一種優化的測定方法,可用于通過熒光顯微鏡檢查高爾基體的形態。




激發: Cy3/TRITC濾波片組
發射: Cy3/TRITC濾波片組
推薦孔板: 黑色透明










向GGR-神經酰胺(組分A)中加入100 µL DMSO(組分C)制成GGR169-神經酰胺原液(100X)。
將10 µL GGR169-神經酰胺原液(100X)添加到990 µL染色緩沖液(組分B)中,制成GGR169-神經酰胺工作液。
可選:向1 mL GGR169-神經酰胺工作溶液中加入10 µL Hoechst 33342(組分D)以進行核染色。在帶有DAPI濾光片組的熒光顯微鏡下觀察。



  1. 接種并根據需要處理細胞。
  2. 將100 µL GGR169-神經酰胺工作溶液直接添加到細胞培養基中。
  3. 在室溫或37°C下孵育15至30分鐘。
  4. 除去GGR169-神經酰胺工作溶液,并用DPBS或您選擇的緩沖液清洗一次。
  5. 加入100 µL /孔的染色緩沖液(組分B)。
  6. 在裝有Cy3濾光片的熒光顯微鏡下觀察。 



圖1.HeLa細胞中GGR169神經酰胺高爾基染色的熒光圖像。在37°C下,用100μL具有Hoechst 33342的GGR169-神經酰胺工作溶液對細胞染色20分鐘。



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Cell Navigator?TMR神經酰胺高爾基體染色試劑盒*紅色熒光*.pdf