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Cell Meter 固定化細胞和組織TUNEL細胞凋亡測定試劑盒*綠色熒光*

貨號22851存儲條件 在零下15度以下保存, 避免光照
規格25 Tests價格4884
Ex (nm)498Em (nm)517
分子量溶劑
產品詳細介紹


簡要概述

產品基本信息

貨號:22851

產品名稱:Cell Meter 固定化細胞和組織TUNEL細胞凋亡測定試劑盒*綠色熒光*

規格:25 Tests

儲存條件:-15℃避光防潮

保質期:12個月

 

產品物理化學光譜特性

Ex(nm):498

Em(nm):517

 

適用儀器


流式細胞儀  
激發: 488nm激光
發射: 530/30nm濾波片
通道: FITC通道
熒光顯微鏡  
激發: FITC濾波片組
發射: FITC濾波片組
推薦孔板: 黑色透明

 

產品介紹

Cell Meter 固定化細胞和組織TUNEL細胞凋亡檢測試劑盒提供了一種強大熒光檢測工具,可方便地檢測由凋亡引起的DNA片段化。該檢測方法是一種非放射性,簡單,準確和快速的方法,可通過對DNA片段進行成像來檢測固定細胞和組織中的細胞凋亡。TUNEL分析使用末端脫氧核苷酸轉移酶(TdT)催化在片段化DNA的3'-羥基末端摻入熒光素12-dUTP。通過熒光顯微鏡或流式細胞儀(用530/30 nm發射濾光片在488 nm激發)分析熒光素標記的DNA。該試劑盒可用于檢測固定細胞和福爾馬林固定的石蠟包埋的組織切片的凋亡。百螢生物是AAT Bioquest的中國代理商,為您提供最優質的Cell Meter 固定化細胞和組織TUNEL細胞凋亡測定試劑盒。 

 

圖示

 

圖1.固定HeLa細胞,并在37°C下用或不使用DNAse處理60分鐘。然后將細胞用Cell Meter TUNEL細胞凋亡檢測試劑盒染色。DNA鏈斷裂在DNAse處理的細胞中顯示出強烈的熒光染色。使用FITC濾光片組通過熒光顯微鏡檢測信號。

 

 

參考文獻

Exosomes derived from adipose tissue, bone marrow, and umbilical cord blood for cardioprotection after myocardial infarction.
Authors: Xu, Huiyu and Wang, Zhongchao and Liu, Longmei and Zhang, Baoxia and Li, Bao
Journal: Journal of cellular biochemistry (2020): 2089-2102

Improvement of in situ Follicular Activation and Early Development in Cryopreserved Human Ovarian Cortical Tissue by Co-Culturing with Mesenchymal Stem Cells.
Authors: Hosseini, Marzieh and Salehpour, Saghar and Ghaffari Novin, Marefat and Shams Mofarahe, Zahra and Abdollahifar, Mohammad-Amin and Piryaei, Abbas
Journal: Cells, tissues, organs (2020): 1-11

Brain tissue saving effects by single-dose intralesional administration of Neuroprotectin D1 on experimental focal penetrating brain injury in rats.
Authors: Berg, Rand Wilcox Vanden and Davidsson, Johan and Lidin, Erik and Angéria, Maria and Risling, Mårten and Günther, Mattias
Journal: Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia (2019): 227-233

Cathepsin K Knockout Exacerbates Haemorrhagic Transformation Induced by Recombinant Tissue Plasminogen Activator After Focal Cerebral Ischaemia in Mice.
Authors: Zhao, Rong and He, Xin-Wei and Shi, Yan-Hui and Liu, Yi-Sheng and Liu, Feng-Di and Hu, Yue and Zhuang, Mei-Ting and Feng, Xiao-Yan and Zhao, Lei and Zhao, Bing-Qiao and Liu, Hui-Qin and Shi, Guo-Ping and Liu, Jian-Ren
Journal: Cellular and molecular neurobiology (2019): 823-831

Classification, Scoring, and Quantification of Cell Death in Tissue Sections.
Authors: Janke, Laura J and Ward, Jerrold M and Vogel, Peter
Journal: Veterinary pathology (2019): 33-38

Comparison between Slow Freezing and Vitrification for Human Ovarian Tissue Cryopreservation and Xenotransplantation.
Authors: Lee, Sanghoon and Ryu, Ki-Jin and Kim, Boram and Kang, Dahyeon and Kim, Yoon Young and Kim, Tak
Journal: International journal of molecular sciences (2019)

Correlation of MiR-152 expression with VEGF expression in placental tissue of preeclampsia rat and its influence on apoptosis of trophoblast cells.
Authors: Zhang, L and Yuan, J-M and Zhao, R-H and Wang, L-M and Tu, Z-B
Journal: European review for medical and pharmacological sciences (2019): 3553-3560

Effect of cryopreservation techniques on proliferation and apoptosis of cultured equine ovarian tissue.
Authors: Gastal, G D A and Aguiar, F L N and Ishak, G M and Cavinder, C A and Willard, S T and Ryan, P L and Feugang, J M and Gastal, E L
Journal: Theriogenology (2019): 88-94

Exosomal miR-320d derived from adipose tissue-derived MSCs inhibits apoptosis in cardiomyocytes with atrial fibrillation (AF).
Authors: Liu, Lina and Zhang, Haoran and Mao, Hongyu and Li, Xiaohong and Hu, Yamin
Journal: Artificial cells, nanomedicine, and biotechnology (2019): 3976-3984

IL-10 inhibits apoptosis in brain tissue around the hematoma after ICH by inhibiting proNGF.
Authors: Song, L and Xu, L-F and Pu, Z-X and Wang, H-H
Journal: European review for medical and pharmacological sciences (2019): 3005-3011



說明書
Cell Meter 固定化細胞和組織TUNEL細胞凋亡測定試劑盒*綠色熒光*.pdf


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