Buccutite FOL,馬來酰亞胺 [FOLM]

貨號5356存儲條件 在零下15度以下保存, 避免光照
規格2 umoles價格2340
Ex (nm)Em (nm)
分子量523.63溶劑DMSO
產品詳細介紹


簡要概述

產品基本信息

貨號:5356

產品名稱:Buccutite FOL,馬來酰亞胺 [FOLM]

規格:2 umoles

儲存條件:-15℃避光防潮

保質期:12個月

 

產品物理化學光譜特性

外觀:固體

分子量:523.63

溶劑:DMSO

 

產品介紹

Buccutite 交聯技術提供了最方便、最有效的交聯方法,以高共軛產率連接兩個生物分子。該方法使用一對交聯劑:Buccutite MTA 和 Buccutite FOL。 MTA 被添加到一個分子中,而 FOL 被添加到另一個分子中。通過在中性條件下混合 Molecule-1-Buccutite MTA 和 Molecule-2-Buccutite FOL 來引發交聯反應。Buccutite FOL 馬來酰亞胺 (FOLM) 的使用方式與廣泛用于交聯蛋白質的 SMCC 相同。 FOLM 的一端(通過馬來酰亞胺)與還原抗體(通過 TCEP 或 DTT)中發現的半胱氨酸硫醇 (-SH) 反應,以產生 FOL 修飾的還原抗體。 Buccutite 交聯反應在極其溫和和中性的條件下發生,不需要任何催化劑,而 SMCC 交聯需要高濃度的蛋白質。此外,SMCC 修飾的蛋白質極不穩定,通常會自我反應,因為蛋白質通常同時含有胺和硫醇基團,這會導致大量的同交聯。Buccutite 交聯反應在極其溫和和中性的條件下發生,無需任何催化劑,它比SMCC更加穩定高效。

 

參考文獻

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說明書
Buccutite FOL,馬來酰亞胺 [FOLM].pdf


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