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iFluor 647小麥胚芽凝集素(WGA)綴合物

貨號25559存儲條件 在零下15度以下保存, 避免光照
規格1 mg價格1176
Ex (nm)656Em (nm)670
分子量N/A溶劑Water
產品詳細介紹


簡要概述

麥胚凝集素(WGA)是一種與N-乙酰-D-葡萄糖胺和唾液酸結合的凝集素。它是目前研究最多、應用最廣泛的一類凝集素。由于WGA與糖綴合物結合,其衍生物和綴合物被廣泛用于標記細胞膜和纖維化瘢痕組織,用于熒光成像和分析。WGA的糖結合特異性與β-1,4-GlcNAc-連接殘基的序列,即幾丁質酶有關。每個單體包含兩個相同的非相互作用的結合位點,它們與3或4β-1,4-GlcNAc單元互補。在所檢測的單糖中,只有GlcNAc與WGA結合。甘露聚糖不結合,而GalNAc只能弱結合。WGA與含有Galβ(1--4)GlcNAcβ(1--3)重復序列(即聚乳糖胺型聚糖)的大寡糖中的內部GlcNAc殘基具有高親和力結合。N-乙酰神經氨酸只參與與WGA的低親和力相互作用。WGA顯示了復雜的糖類特異性模式,可用于復雜碳水化合物的結構分析。iFluor 647小麥胚芽凝集素(WGA)綴合物可能是最亮的WGA綴合物。它表現出了iFluor的明亮和綠色熒光。iFluor 647小麥胚芽凝集素(WGA)綴合物與唾液酸和N-乙酰氨基葡萄糖殘基結合,如AF647 WGA結合物那樣。百螢生物是AAT Bioquest的中國代理商,為您提供最優質的iFluor 647小麥胚芽凝集素(WGA)綴合物。 

點擊查看光譜

 

適用儀器


熒光顯微鏡  
激發: Cy5濾波片
發射: Cy5濾波片
推薦孔板: 黑色透明


產品說明書

樣品分析方案

溶液配制

儲備溶液配制 

iFluor 647 小麥胚芽凝集素 (WGA) 綴合物儲備液 (200X)

將 500 µL ddH2O 添加到粉末小瓶中,制成 2 mg/mL 的儲備溶液。 注意:重新配制的綴合物溶液可在 2-8 °C 下短期儲存,或在 -20 °C 下長期儲存。

工作溶液配制 

iFluor 647-小麥胚芽凝集素 (WGA) 綴合物??工作溶液 (1X)

將 5 μL 200X WGA 儲備溶液添加到 1 mL HHBS 緩沖液中。
注意:不同細胞系的優化染色濃度可能不同。 活細胞的推薦起始濃度為 5-10 µg/mL。

 

操作步驟

1.活細胞染色
1.1 用 HHBS 緩沖液清洗細胞兩次。
1.2 添加 100 µL iFluor 647-WGA 工作溶液。
1.3 將細胞與 WGA 工作溶液在 37°C 下孵育 10-30 分鐘。
1.4 用 HHBS 緩沖液清洗細胞兩次。
1.5 使用 Cy5 濾光片組在熒光顯微鏡上成像細胞。

 

2.固定細胞染色
2.1 在PBS中使用4%的甲醛固定細胞。
注意:對于固定細胞膜染色,建議在沒有透化步驟的情況下進行染色。固定后透化步驟會導致細胞內區室染色,如高爾基體和內質網 (ER) 結構染色。
2.2 添加 100 µL iFluor 647-WGA 工作溶液。
2.3 在室溫下用 WGA 工作溶液孵育細胞 10-30 分鐘。
2.4 用 HHBS 緩沖液清洗細胞兩次。
2.5 使用 Cy5 濾光片組在熒光顯微鏡上成像細胞。

 

圖示

圖 1. 使用 iFluor 647-小麥胚芽凝集素 (WGA) 綴合物??以 5 µg/mL的濃度在活細胞(Hela細胞) 染色 30 分鐘,然后用 Hoechst 33342(Cat# 17535)染色。最后使用帶Cy5和DAPI濾波片組的熒光顯微鏡成像。

 

參考文獻

Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of concanavalin A- and wheat germ agglutinin-reactive glycans mark seminal prostasome populations from normozoospermic and oligozoospermic men.
Authors: Jankovi?, Tamara and Go?, Sanja and Miti?, Ninoslav and Danilovi? Lukovi?, Jelena and Jankovi?, Miroslava
Journal: Upsala journal of medical sciences (2020): 10-18

Succinylated Wheat Germ Agglutinin Colocalizes with the Toxoplasma gondii Cyst Wall Glycoprotein CST1.
Authors: Guevara, Rebekah B and Fox, Barbara A and Bzik, David J
Journal: mSphere (2020)

Wheat germ agglutinin is a biomarker of whole grain content in wheat flour and pasta.
Authors: Killilea, David W and McQueen, Rebecca and Abegania, Judi R
Journal: Journal of food science (2020): 808-815

Wheat germ agglutinin is involved in the protective action of 24-epibrassinolide on the roots of wheat seedlings under drought conditions.
Authors: Avalbaev, Azamat and Bezrukova, Marina and Allagulova, Chulpan and Lubyanova, Alsu and Kudoyarova, Guzel and Fedorova, Kristina and Maslennikova, Dilara and Yuldashev, Ruslan and Shakirova, Farida
Journal: Plant physiology and biochemistry : PPB (2020): 420-427

Wheat germ agglutinin liposomes with surface grafted cyclodextrins as bioadhesive dual-drug delivery nanocarriers to treat oral cells.
Authors: Wijetunge, Sashini S and Wen, Jianchuan and Yeh, Chih-Ko and Sun, Yuyu
Journal: Colloids and surfaces. B, Biointerfaces (2020): 110572

Wheat germ agglutinin-conjugated fluorescent pH sensors for visualizing proton fluxes.
Authors: Zhang, Lejie and Zhang, Mei and Bellve, Karl and Fogarty, Kevin E and Castro, Maite A and Brauchi, Sebastian and Kobertz, William R
Journal: The Journal of general physiology (2020)

Bacterial expression, purification and biophysical characterization of wheat germ agglutinin and its four hevein-like domains.
Authors: Leyva, Eduardo and Medrano-Cerano, Jorge L and Cano-Sánchez, Patricia and López-González, Itzel and Gómez-Velasco, Homero and Del Río-Portilla, Federico and García-Hernández, Enrique
Journal: Biopolymers (2019): e23242

Enhanced anti-colon cancer efficacy of 5-fluorouracil by epigallocatechin-3- gallate co-loaded in wheat germ agglutinin-conjugated nanoparticles.
Authors: Wang, Ruoning and Huang, Jinyu and Chen, Jian and Yang, Mengmeng and Wang, Honglan and Qiao, Hongzhi and Chen, Zhipeng and Hu, Lihong and Di, Liuqing and Li, Junsong
Journal: Nanomedicine : nanotechnology, biology, and medicine (2019): 102068

Targeted delivery of etoposide, carmustine and doxorubicin to human glioblastoma cells using methoxy poly(ethylene glycol)?poly(ε?caprolactone) nanoparticles conjugated with wheat germ agglutinin and folic acid.
Authors: Kuo, Yung-Chih and Chang, Yu-Hsuan and Rajesh, Rajendiran
Journal: Materials science & engineering. C, Materials for biological applications (2019): 114-128

Towards the preparation of synthetic outer membrane vesicle models with micromolar affinity to wheat germ agglutinin using a dialkyl thioglycoside.
Authors: Fayolle, Dimitri and Berthet, Nathalie and Doumeche, Bastien and Renaudet, Olivier and Strazewski, Peter and Fiore, Michele
Journal: Beilstein journal of organic chemistry (2019): 937-946

 

相關產品

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iFluor 555小麥胚芽凝集素(WGA)綴合物 Cat#25539
iFluor 594小麥胚芽凝集素(WGA)綴合物 Cat#25550
iFluor 488小麥胚芽凝集素(WGA)綴合物 Cat#25530


說明書
iFluor 647小麥胚芽凝集素(WGA)綴合物.pdf


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