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ProLite 橙色蛋白凝膠染料* 5000X *

貨號18001存儲條件 在零下15度以下保存, 避免光照
規格1 ml價格2340
Ex (nm)484Em (nm)586
分子量486.72溶劑Water
產品詳細介紹


簡要概述

Prolite Orange是一種替代性蛋白質染料,可用于替代SYPRO Orange蛋白凝膠染料(SYPRO是ThermoFisher的商標)。 Prolite Orange是一種靈敏的即用型熒光染料,可在一維凝膠中檢測總蛋白。 Prolite Orange的靈敏度比傳統的銀染技術更好。 可以使用標準的UV或藍光透射照明器或包含適當濾鏡或激光的成像設備查看染色的蛋白質。 熒光染色快速且高度靈敏,可檢測蛋白質電泳凝膠和膜中的總蛋白質。 

點擊查看光譜



產品說明書

樣品分析方案

儲存

存放在-20°C避光的地方。 按建議存放時,產品自收到之日起至少穩定12個月。 可用醋酸或緩沖液稀釋的ProLite Orange可在4°C的玻璃或塑料瓶中保存三個月,避光。 打開之前,應先將每個小瓶加熱至室溫,然后在微量離心機中短暫離心,以將DMSO溶液沉積在小瓶底部。 如果存在染料顆粒,請短暫超聲處理試管或劇烈渦旋試管。

 

工作溶液配制

ProLite Orange工作溶液(5000X)
用7.5%(v / v)乙酸稀釋5000X ProLite Orange儲備溶液,制成1X ProLite Orange儲備溶液,并劇烈混合。
注意:工作溶液最多可以重復使用四次。 但是,我們觀察到第二次重用后響應開始明顯減少。 強烈建議使用全新的工作溶液以獲得最佳效果。

 

操作步驟

建議使用以下方案,并且可以將其用作準則。但是,可能需要進行一些比較才能確定哪一種可以更好地滿足您的需求。

 

電泳后染色蛋白

1.運行凝膠。
2.將工作溶液倒入一個小的塑料皿中。
注意:對于一到兩個標準尺寸的小凝膠,請使用約50 mL的工作溶液。對于較大的凝膠,請使用500至700 mL的工作溶液。
注意:確保添加足夠的工作量以完全浸沒凝膠。
3.將凝膠放入工作溶液中。
注意:用鋁箔蓋住容器,以防止染料受光。
4.在室溫下輕輕攪拌凝膠10至60分鐘。
5.用7.5%的乙酸短暫沖洗。
6.可以在標準的300 nm紫外線透射儀或藍光透射儀上觀察凝膠。
7.脫色:大部分凝膠可通過在0.1%Tween®20中孵育過夜來脫色?;蛘?,在7.5%乙酸的多種變化中孵育最終將去除所有污漬。

 

參考文獻

Fluorescent thermal shift-based method for detection of NF-κB binding to double-stranded DNA.
Authors: Leitner, Peter D and Vietor, Ilja and Huber, Lukas A and Valovka, Taras
Journal: Scientific reports (2021): 2331

Ligand binding to a humanized anti-cocaine mAb measured by dye absorption spectroscopy.
Authors: Kirley, Terence L and Norman, Andrew B
Journal: Biochemical and biophysical research communications (2021): 93-98

Thermal Shift Assay for Exploring Interactions Between Fatty Acid-Binding Protein and Inhibitors.
Authors: Hao, Jiaqing
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 395-409

Thermal shift assay to probe melting of thrombin, fibrinogen, fibrin monomer, and fibrin: Gly-Pro-Arg-Pro induces a fibrin monomer-like state in fibrinogen.
Authors: Crossen, J and Diamond, S L
Journal: Biochimica et biophysica acta. General subjects (2021): 129805

A novel differential scanning fluorimetry analysis of a humanized anti-cocaine mAb and its ligand binding characteristics.
Authors: Kirley, Terence L and Norman, Andrew B and Wetzel, Hanna N
Journal: Journal of immunological methods (2020): 112676

Intrinsic Differential Scanning Fluorimetry for Fast and Easy Identification of Adeno-Associated Virus Serotypes.
Authors: Rieser, Ruth and Penaud-Budloo, Magalie and Bouzelha, Mohammed and Rossi, Axel and Menzen, Tim and Biel, Martin and Büning, Hildegard and Ayuso, Eduard and Winter, Gerhard and Michalakis, Stylianos
Journal: Journal of pharmaceutical sciences (2020): 854-862

SYPRO Orange - a new gold standard amyloid probe.
Authors: Mora, Aruna K and Nath, Sukhendu
Journal: Journal of materials chemistry. B (2020): 7894-7898

nanoDSF: In vitro Label-Free Method to Monitor Picornavirus Uncoating and Test Compounds Affecting Particle Stability.
Authors: Real-Hohn, Antonio and Groznica, Martin and Löffler, Nadine and Blaas, Dieter and Kowalski, Heinrich
Journal: Frontiers in microbiology (2020): 1442

Destructive twisting of neutral metalloproteases: the catalysis mechanism of the Dispase autolysis-inducing protein from Streptomyces mobaraensis DSM 40487.
Authors: Fiebig, David and Storka, Juliana and Roeder, Markus and Meyners, Christian and Schmelz, Stefan and Blankenfeldt, Wulf and Scrima, Andrea and Kolmar, Harald and Fuchsbauer, Hans-Lothar
Journal: The FEBS journal (2018): 4246-4264

Evaluation of fluorescent dyes to measure protein aggregation within mammalian cell culture supernatants.
Authors: Oshinbolu, Sheun and Shah, Rachana and Finka, Gary and Molloy, Mike and Uden, Mark and Bracewell, Daniel G
Journal: Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986) (2018): 909-917



說明書
ProLite 橙色蛋白凝膠染料* 5000X *.pdf


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