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細胞核染料Hoechst 33342 CAS 23491-52-3

貨號17530存儲條件 在零下15度以下保存, 避免光照
規格100 mg價格1236
Ex (nm)352Em (nm)454
分子量561.93溶劑Water
產品詳細介紹


簡要概述

產品基本信息

貨號:17530

產品名稱:Hoechst 33342細胞核染料

規格:100mg

儲存條件:-20℃干燥

保質期:12個月

 

產品物理化學光譜特性

分子量:561.93

外觀:亮黃色粉末

溶劑:水或DMSO

激發波長(nm):350

發射波長(nm):461

 

適用儀器


熒光顯微鏡  
激發: 350nm
發射: 461nm
推薦孔板: 黑色透明

 

產品介紹

Hoechst 33342細胞核染料,用于在熒光顯微鏡中標記DNA。它們也常用于可視化細胞核和線粒體的染色??蒲兄型ǔJ褂玫膬煞N分別為:Hoechst 33258和Hoechst 33342。 兩種染料都被約350nm的紫外光激發,并且都發射藍/青色熒光,發射最大值為461nm。 Hoechst染色劑可以與活細胞或固定細胞一起使用,并且通常用作另一種核酸染色劑DAPI的替代物。 它們之間的關鍵區別在于Hoechst 33342的額外乙基使其更具親脂性,因此更容易穿過完整的細胞膜。在某些應用中,Hoechst 33258的滲透性明顯低于Hoeschst 33342。這些染料也可用于通過繪制標準的發射 - 含量曲線來檢測樣品的DNA的含量。百螢生物是AAT Bioquest的中國代理商,為您提供最優質的Hoechst 33342細胞核染料。 

點擊查看光譜



產品說明書

染色細胞分析方案       

        以下程序適用于大多數細胞類型。 生長培養基,細胞密度,細胞類型和其他因素可能影響染色。 玻璃器皿上的殘留洗滌劑也可能影響許多生物染色,導致在沒有細胞存在的溶液中出現明亮染色的物質。

        通過離心沉淀細胞并將細胞重懸于緩沖鹽溶液或培養基中,經過測試染料的最佳結合pH為7.4。培養物中的貼壁細胞可以在蓋玻片上或細胞培養孔中原位染色。Hoechst的染色濃度為0.5-5nM,并將其孵育15至60分鐘。在最初的實驗中,最好在整個建議范圍內嘗試幾種染料濃度,以確定產生最佳染色的濃度。

 

參考文獻

Amine terminated G-6 PAMAM dendrimer and its interaction with DNA probed by Hoechst 33258
Authors: Choi YS, Cho TS, Kim JM, Han SW, Kim SK.
Journal: Biophys Chem (2006): 142

Design of new bidentate ligands constructed of two Hoechst 33258 units for discrimination of the length of two A3T3 binding motifs
Authors: Tanada M, Tsujita S, Sasaki S.
Journal: J Org Chem (2006): 125

DNA sequence recognition by Hoechst 33258 conjugates of hairpin pyrrole/imidazole polyamides
Authors: Correa BJ, Canzio D, Kahane AL, Reddy PM, Bruice TC.
Journal: Bioorg Med Chem Lett (2006): 3745

Recognition of B-DNA by neomycin--Hoechst 33258 conjugates
Authors: Willis B, Arya DP.
Journal: Biochemistry (2006): 10217

The Hoechst 33258 Covalent Dimer Covers a Total Turn of the Double-stranded DNA
Authors: Streltsov SA, Gromyko AV, Oleinikov VA, Zhuze AL.
Journal: J Biomol Struct Dyn (2006): 285

Activity of Hoechst 33258 against Pneumocystis carinii f. sp. muris, Candida albicans, and Candida dubliniensis
Authors: Disney MD, Stephenson R, Wright TW, Haidaris CG, Turner DH, Gigliotti F.
Journal: Antimicrob Agents Chemother (2005): 1326

Association of the minor groove binding drug Hoechst 33258 with d(CGCGAATTCGCG)2: volumetric, calorimetric, and spectroscopic characterizations
Authors: Han F, Taulier N, Chalikian TV.
Journal: Biochemistry (2005): 9785

Hoechst 33258 as a pH-sensitive probe to study the interaction of amine oxide surfactants with DNA
Authors: Goracci L, Germani R, Savelli G, Bassani DM.
Journal: Chembiochem (2005): 197

Hydration changes in the association of Hoechst 33258 with DNA
Authors: Kiser JR, Monk RW, Smalls RL, Petty JT.
Journal: Biochemistry (2005): 16988

Recognition of a 10 base pair sequence of DNA and stereochemical control of the binding affinity of chiral hairpin polyamide-Hoechst 33258 conjugates
Authors: Reddy PM, Toporowski JW, Kahane AL, Bruice TC.
Journal: Bioorg Med Chem Lett (2005): 5531

 

相關產品

產品名稱 貨號
細胞核染料Hoechst 33342 20mM水溶液 Cat#17535


說明書
細胞核染料Hoechst 33342 CAS 23491-52-3.pdf


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