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DAPI(4',6-二脒基-2-苯基吲哚二鹽酸鹽)*10mM水溶液*

貨號17507存儲條件 在零下15度以下保存, 避免光照
規格2 mL價格984
Ex (nm)359Em (nm)457
分子量350.25溶劑Water
產品詳細介紹


簡要概述

產品基本信息

貨號:17507

產品名稱:DAPI[4,6-二脒基-2-苯基吲哚,二鹽酸鹽]* 10 mM水溶液*

規格:2ml

儲存條件:保存在冰箱-20℃干燥

保質期:6個月

 

產品物理化學光譜特性

分子量:350.25

溶劑:水

Ex(nm):358

Em(nm):461

 

產品介紹

DAPI是一種與DNA結合強烈的熒光染色劑。它廣泛用于熒光顯微鏡。由于DAPI通過完整的細胞膜,它可用于除固定細胞外的活細胞染色。對于熒光顯微鏡,DAPI被紫外線激發。當與雙鏈DNA結合時,其最大吸收在358nm,其發射最大值在461nm。DAPI的一個缺點是其排放相當廣泛。DAPI也與RNA結合,但是它不像它與DNA結合時那樣強烈地發熒光。 當與RNA結合時,其發射偏移至約500nm .DAPI的藍色發射便于多重測定,因為DAPI與熒光素和綠色熒光蛋白(GFP)等綠色熒光分子或紅色熒光染料之間的熒光重疊非常少。除了標記細胞核外,DAPI還用于檢測細胞培養物中的支原體或病毒DNA。

點擊查看光譜

 

參考文獻

Astrocytic expression of the CXCL12 receptor, CXCR7/ACKR3 is a hallmark of the diseased, but not developing CNS
Authors: Malte Puchert, Fabian Pelkner, Gregor Stein, Doychin N Angelov, Johannes Boltze, Daniel-Christoph Wagner, Francesca Odoardi, Alexander Flügel, Wolfgang J Streit, Jürgen Engele
Journal: Molecular and Cellular Neuroscience (2017)

Cube-shaped theranostic paclitaxel prodrug nanocrystals with surface functionalization of SPC and MPEG-DSPE for imaging and chemotherapy
Authors: Fuqiang Guo, Jiajia Shang, Hai Zhao, Kangrong Lai, Yang Li, Zhongxiong Fan, Zhenqing Hou, Guanghao Su
Journal: Colloids and Surfaces B: Biointerfaces (2017)

Legionella pneumophila infection activates bystander cells differentially by bacterial and host cell vesicles
Authors: Anna Lena Jung, Christina Elena Herkt, Christine Schulz, Kathrin Bolte, Kerstin Seidel, Nicoletta Scheller, Alexandra Sittka-Stark, Wilhelm Bertrams, Bernd Schmeck
Journal: Scientific Reports (2017)

Peptides mimicking viral proteins of porcine circovirus type 2 were profiled by the spectrum of mouse anti-PCV2 antibodies
Authors: Ling-Chu Hung, Cheng-Yao Yang, Ivan-Chen Cheng
Journal: BMC immunology (2017): 25

Poly (ADP-ribose) polymerase inhibitors activate the p53 signaling pathway in neural stem/progenitor cells
Authors: Akiko Okuda, Suguru Kurokawa, Masanori Takehashi, Aika Maeda, Katsuya Fukuda, Yukari Kubo, Hyuma Nogusa, Tomoka Takatani-Nakase, Shujiro Okuda, Kunihiro Ueda
Journal: BMC neuroscience (2017): 14

Apoptotic effect of the selective PPARβ/δ agonist GW501516 in invasive bladder cancer cells
Authors: Adeline Péchery, Sylvie Fauconnet, Hugues Bittard, Isabelle Lascombe
Journal: Tumor Biology (2016): 1--14

Carbon nanotube--Protamine hybrid: Evaluation of DNA cell penetration
Authors: Cécile Caoduro, Raoudha Kacem, Khaoula Boukari, Fabien Picaud, Claire-Hélène Brachais, David Monchaud, Christophe Borg, Hatem Boulahdour, Tijani Gharbi, Régis Delage-Mourroux
Journal: Carbon (2016): 742--752

Identification of epigenetic factors regulating the mesenchyme to epithelium transition by RNA interference screening in breast cancer cells
Authors: Jean-Marc Gregoire, Laurence Fleury, Clara Salazar-Cardozo, Frédéric Alby, Véronique Masson, Paola Barbara Arimondo, Frédéric Ausseil
Journal: BMC cancer (2016): 700

Legionella pneumophila-Derived Outer Membrane Vesicles Promote Bacterial Replication in Macrophages
Authors: Anna Lena Jung, Cornelia Stoiber, Christina E Herkt, Christine Schulz, Wilhelm Bertrams, Bernd Schmeck
Journal: PLoS Pathog (2016): e1005592

Redox-Sensitive Citronellol--Cabazitaxel Conjugate: Maintained in Vitro Cytotoxicity and Self-Assembled as Multifunctional Nanomedicine
Authors: Peng Xue, Dan Liu, Jing Wang, Na Zhang, Jiahua Zhou, Lin Li, Weiling Guo, Mengchi Sun, Xiangfei Han, Yongjun Wang
Journal: Bioconjugate chemistry (2016): 1360--1372

 

相關產品

產品名稱 貨號
DAPI(4',6-二脒基-2-苯基吲哚二鹽酸鹽)CAS 28718-90-3 Cat#17510


說明書
DAPI(4',6-二脒基-2-苯基吲哚二鹽酸鹽)*10mM水溶液*.pdf


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