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trFluor Eu羊抗兔免疫球蛋白(H+L)

貨號16668存儲條件 在零下15度以下保存, 避免光照
規格100 ug價格3840
Ex (nm)346Em (nm)617
分子量~150000溶劑Water
產品詳細介紹


簡要概述

產品基本信息

貨號:16668

產品名稱:trFluor Eu羊抗兔免疫球蛋白(H+L)

規格:100 ug

儲存條件:-15℃避光防潮

保質期:24個月

 

產品物理化學光譜特性

分子量:N/A

溶劑:水

激發波長(nm):346

發射波長(nm):617

 

產品介紹

trFluor Eu羊抗兔免疫球蛋白(H+L)是美國AAT Bioquest生產的熒光標記二抗,存在于細胞,血清或其他生物流體中的許多生物化合物都是天然熒光的,因此,由于要測定的生物分子的自發熒光引起的高背景,使用常規的快速熒光團會嚴重限制測定靈敏度。長壽命熒光團與時間分辨檢測(激發和發射檢測之間的延遲)結合使用,可最大程度地減少即時熒光干擾。我們的trFluor Eu探針可用于需要高靈敏度的測定的時間分辨熒光(TRF)。與更傳統的熒光團(例如Alexa Fluor或花青染料)相比,trFluor Eu探針具有較大的斯托克斯位移和極長的發射半衰期。與其他TRF化合物相比,我們的trFluor Eu探針具有相對較高的穩定性,高發射率和與生物分子連接的能力。當與一抗結合使用時,這種trFluor Eu山羊抗兔IgG(H + L)綴合物通常用作間接免疫熒光染色的第二步試劑。百螢生物是AAT Bioquest的中國代理商,為您提供最優質的trFluor Eu羊抗兔免疫球蛋白(H+L)。 

點擊查看光譜

 

參考文獻

Development of a time-resolved fluorescence resonance energy transfer assay for cyclin-dependent kinase 4 and identification of its ATP-noncompetitive inhibitors
Authors: Lo MC, Ngo R, Dai K, Li C, Liang L, Lee J, Emkey R, Eksterowicz J, Ventura M, Young SW, Xiao SH.
Journal: Anal Biochem (2012): 368

Time-Resolved Fluorescence Resonance Energy Transfer as a Versatile Tool in the Development of Homogeneous Cellular Kinase Assays
Authors: Saville L, Spais C, Mason JL, Albom MS, Murthy S, Meyer SL, Ator MA, Angeles TS, Husten J.
Journal: Assay Drug Dev Technol. (2012)

A homogeneous single-label time-resolved fluorescence cAMP assay
Authors: Martikkala E, Rozwandowicz-Jansen A, Hanninen P, Petaja-Repo U, Harma H.
Journal: J Biomol Screen (2011): 356

Homogeneous time-resolved fluorescence-based assay to screen for ligands targeting the growth hormone secretagogue receptor type 1a
Authors: Leyris JP, Roux T, Trinquet E, Verdie P, Fehrentz JA, Oueslati N, Douzon S, Bourrier E, Lamarque L, Gagne D, Galleyrand JC, M'Kadmi C, Martinez J, Mary S, Baneres JL, Marie J.
Journal: Anal Biochem (2011): 253

Oligomerization of the serotonin(1A) receptor in live cells: a time-resolved fluorescence anisotropy approach
Authors: Paila YD, Kombrabail M, Krishnamoorthy G, Chattopadhyay A.
Journal: J Phys Chem B (2011): 11439

Time-resolved fluorescence resonance energy transfer (TR-FRET) to analyze the disruption of EGFR/HER2 dimers: a new method to evaluate the efficiency of targeted therapy using monoclonal antibodies
Authors: Gaborit N, Larbouret C, Vallaghe J, Peyrusson F, Bascoul-Mollevi C, Crapez E, Azria D, Chardes T, Poul MA, Mathis G, Bazin H, Pelegrin A.
Journal: J Biol Chem (2011): 11337

A time-resolved fluorescence-resonance energy transfer assay for identifying inhibitors of hepatitis C virus core dimerization
Authors: Kota S, Scampavia L, Spicer T, Beeler AB, Takahashi V, Snyder JK, Porco JA, Hodder P, Strosberg AD.
Journal: Assay Drug Dev Technol (2010): 96

Ligand regulation of the quaternary organization of cell surface M3 muscarinic acetylcholine receptors analyzed by fluorescence resonance energy transfer (FRET) imaging and homogeneous time-resolved FRET
Authors: Alvarez-Curto E, Ward RJ, Pediani JD, Milligan G.
Journal: J Biol Chem (2010): 23318

Steady-state and time-resolved fluorescence quenching with transition metal ions as short-distance probes for protein conformation
Authors: Posokhov YO, Kyrychenko A, Ladokhin AS.
Journal: Anal Biochem (2010): 284

Time-resolved FRET fluorescence spectroscopy of visible fluorescent protein pairs
Authors: Visser AJ, Laptenok SP, Visser NV, van Hoek A, Birch DJ, Brochon JC, Borst JW.
Journal: Eur Biophys J (2010): 241

 

相關產品

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trFluor Eu羊抗鼠免疫球蛋白(H+L) Cat#16518
Cy7®羊抗兔免疫球蛋白(H+L) Cat#16872


說明書
trFluor Eu羊抗兔免疫球蛋白(H+L).pdf


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