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蛋白酶體20S熒光底物Suc-LLVY-AMC

貨號13453存儲條件 在零下15度以下保存, 避免光照
規格1 mg價格1236
Ex (nm)341Em (nm)441
分子量763.88溶劑DMSO
產品詳細介紹


簡要概述

蛋白酶體20S熒光底物Suc-LLVY-AMC是美國AAT Bioquest生產的熒光底物,蛋白酶體的主要功能是通過蛋白水解(一種破壞肽鍵的化學反應)降解不需要的或受損的蛋白質。 蛋白酶體降解途徑對于許多細胞過程是必不可少的,包括細胞周期,基因表達的調節和對氧化應激的反應。 該途徑中最常見的蛋白酶體形式是蛋白酶體26S,一種ATP依賴性蛋白水解復合物,其含有一個20S(700-kDa)核心顆粒結構和兩個19S(700-kDa)調節帽。 20S核心包含三種主要的蛋白水解活性,包括胰凝乳蛋白酶樣,胰蛋白酶樣和半胱天冬酶樣活性。 它負責細胞凋亡,DNA修復,內吞作用和細胞周期控制所涉及的關鍵蛋白的分解。

AAT Bioquest提供一組R110底物,用于監測蛋白酶體在不同亞位點的蛋白酶活性,即(i)亞位點:1c,Z-LLE-R110; 2c,Ac-KQL-R110; 5c,Ac-WLA-R110; 1i,Ac-PAL-R110; 2i,Ac-KQL-R110; 5c,Ac-WLA-R110和Suc-LLVY-R110;和5i,Ac-ANW-R110。通過分別使用490nm和520nm的激發和發射波長監測R110隨時間的釋放來測量蛋白酶活性。我們還提供Suc-LLVY-AMC,非熒光基板產生亮藍色熒光AMC產品,其Ex / Em = 351 / 430nm,并且可以使用DAPI濾光片組輕松檢測。通常,R110底物比基于AMC-,AFC-或4-硝基苯胺的底物靈敏得多。百螢生物是AAT Bioquest的中國代理商,為您提供最優質的蛋白酶體20S熒光底物Suc-LLVY-AMC。

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產品說明書

操作方法

以下說明書僅提供指南,實際應根據您的具體需求進行修改。

 

1.在DMSO中制備5至10 mM的儲備溶液。

 

2.準備2X蛋白酶體底物(20至50μM)測定溶液,如下所示:

25至50 L底物儲備液(10 mM)

100升DTT(1M)

400 L EDTA(100 mM)

10mL Hepes Buffer(25mM),pH = 7.4

 

3.用2X熒光蛋白酶體底物測定溶液(來自步驟1)混合等體積的質體標準物或樣品,并在室溫下孵育溶液至少1小時。

 

4.監測熒光使用熒光酶標儀。

 

參考文獻

Calpain inhibition improves erectile function in diabetic mice via upregulating endothelial nitric oxide synthase expression and reducing apoptosis.
Authors: Hao Li, Li-Ping Chen, Tao Wang, Shao-Gang Wang, Ji-Hong Liu
Journal: Asian journal of andrology (2018)

Diabetogenic agent alloxan is a proteasome inhibitor
Authors: Wenjuan Zhou, Lingling Wei, Ting Xiao, Chunyou Lai, Min Peng, Lingli Xu, Xiangwei Luo, Shaoping Deng, Fengxue Zhang
Journal: Biochemical and Biophysical Research Communications (2017): 400--406

Delineation of molecular pathways involved in cardiomyopathies caused by troponin T mutations
Authors: Jennifer E Gilda, Xianyin Lai, Frank A Witzmann, Aldrin V Gomes
Journal: Molecular & Cellular Proteomics (2016): 1962--1981

Diclofenac induces proteasome and mitochondrial dysfunction in murine cardiomyocytes and hearts
Authors: Rajeshwary Ghosh, Sumanta K Goswami, Luis Felipe BB Feitoza, Bruce Hammock, Aldrin V Gomes
Journal: International Journal of Cardiology (2016): 923--935

Advanced-glycation-end-product-induced formation of immunoproteasomes: involvement of RAGE and Jak2/STAT1
Authors: Stefanie Grimm, Christiane Ott, Melanie Hörlacher, Daniela Weber, Annika Höhn, Tilman Grune
Journal: Biochemical Journal (2012): 127--139



說明書
蛋白酶體20S熒光底物Suc-LLVY-AMC.pdf


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